Part:BBa_K4432041:Design

Pseudomonas aeruginosa PhzA2-G2 operon

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 933
  Illegal EcoRI site found at 3555
  Illegal PstI site found at 1076
  Illegal PstI site found at 4560
  Illegal PstI site found at 5109
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 933
  Illegal EcoRI site found at 3555
  Illegal PstI site found at 1076
  Illegal PstI site found at 4560
  Illegal PstI site found at 5109
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 933
  Illegal EcoRI site found at 3555
  Illegal BamHI site found at 276
  Illegal BamHI site found at 1488
  Illegal XhoI site found at 220
  Illegal XhoI site found at 379
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 933
  Illegal EcoRI site found at 3555
  Illegal PstI site found at 1076
  Illegal PstI site found at 4560
  Illegal PstI site found at 5109
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 933
  Illegal EcoRI site found at 3555
  Illegal PstI site found at 1076
  Illegal PstI site found at 4560
  Illegal PstI site found at 5109
  Illegal NgoMIV site found at 142
  Illegal NgoMIV site found at 1157
  Illegal NgoMIV site found at 1304
  Illegal NgoMIV site found at 1322
  Illegal NgoMIV site found at 1326
  Illegal NgoMIV site found at 2130
  Illegal NgoMIV site found at 2304
  Illegal AgeI site found at 5398
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 5541
  Illegal BsaI.rc site found at 268
  Illegal BsaI.rc site found at 792
  Illegal BsaI.rc site found at 2240
  Illegal BsaI.rc site found at 4617
  Illegal BsaI.rc site found at 5023
  Illegal BsaI.rc site found at 5050
  Illegal SapI.rc site found at 4037
  

Design Notes

nucleotides 2 070 685 to 2 076 958 of Pseudomonas aeruginosa PAO1 genome (GenBank AE004091.2).

Source

PCR amplification from Pseudomonas aeruginosa PAO1 genomic DNA